Experimental antibodies for Parkinson's, Alzheimer's may cause harmful inflammation

 A team led by scientists at Scripps Research has made a discovery suggesting that experimental antibody therapies for Parkinson's and Alzheimer's have an unintended adverse effect--brain inflammation--that may have to be countered if these treatments are to work as intended.

Experimental antibody treatments for Parkinson's target abnormal clumps of the protein alpha-synuclein, while experimental antibody treatments for Alzheimer's target abnormal clumps of amyloid beta protein. Despite promising results in mice, these potential treatments so far have not seen much success in clinical trials.

"Our findings provide a possible explanation for why antibody treatments have not yet succeeded against neurodegenerative diseases," says study co-senior author Stuart Lipton, MD, PhD, Step Family Foundation Endowed Chair in the Department of Molecular Medicine and founding co-director of the Neurodegeneration New Medicines Center at Scripps Research.

Lipton, also a clinical neurologist, says the study marks the first time that researchers have examined antibody-induced brain inflammation in a human context. Prior research was conducted in mouse brains, whereas the current study used human brain cells.

The study will appear in the Proceedings of the National Academy of Sciences of the United States of America during the week of March 29.

An approach that may need tweaking

Neurodegenerative diseases such as Alzheimer's and Parkinson's afflict more than 6 million Americans. These diseases generally feature the spread of abnormal protein clusters in the brain, with different mixes of proteins predominating in different disorders.

An obvious treatment strategy, which pharmaceutical companies began to pursue in the 1990s, is to inject patients with antibodies that specifically target and clear these protein clusters, also called aggregates.

The aggregates have included not only the large clusters that pathologists observe in patients' brains at autopsy, but also the much smaller and harder-to-detect clusters called oligomers that are now widely considered the most harmful to the brain.

Exactly how these protein clusters damage brain cells is an area of active investigation, but inflammation is a likely contributing factor. In Alzheimer's, for example, amyloid beta oligomers are known to shift brain immune cells called microglia to an inflammatory state in which they can damage or kill healthy neurons nearby.

Surprise finding

Lipton and colleagues were studying alpha synuclein oligomers' ability to trigger this inflammatory state when they encountered a surprise finding: While the oligomers on their own triggered inflammation in microglia derived from human stem cells, adding therapeutic antibodies made this inflammation worse, not better. The team traced this effect not to the antibodies per se but to the complexes formed with antibodies and their alpha synuclein targets.

Amyloid beta aggregates often co-exist with the alpha synuclein aggregates seen in Parkinson's brains, just as alpha synuclein often co-exists with amyloid beta in Alzheimer's brains.

In the study, the researchers added amyloid beta oligomers to their mix, mimicking what would happen in a clinical case, and found that it worsened inflammation. Adding anti-amyloid beta antibodies worsened it even further. They found that both alpha synuclein antibodies and amyloid beta antibodies made inflammation worse when they successfully hit their oligomer targets.

Lipton notes that virtually all prior studies of the effects of experimental antibody treatments were done with mouse microglia, whereas the key experiments in this study were done with human-derived microglia--either in cell cultures or transplanted into the brains of mice whose immune system had been engineered to accommodate the human microglia.

"We see this inflammation in human microglia, but not in mouse microglia, and thus this massive inflammatory effect may have been overlooked in the past," Lipton says.

Microglial inflammation of the kind observed in the study, he adds, could conceivably reverse any benefit of antibody treatment in a patient without being clinically obvious.

Lipton says that he and his colleagues have recently developed an experimental drug that may be able to counter this inflammation and thereby restore any benefit of antibody treatment in the human brain. They are actively working on this now.

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The lead author of the study was Dorit Truder, PhD, a postdoctoral fellow in the Lipton laboratory. Other senior authors were Rajesh Ambasudhan, PhD, an adjunct assistant professor at Scripps Research; Michael Karin, PhD, a professor at the UC San Diego School of Medicine; and Nicholas Schork, PhD, a professor at the Translational Genomics Institute in Phoenix and adjunct professor at UC San Diego and Scripps Research.

"Soluble α-synuclein/antibody complexes activate the NLRP3 inflammasome in hiPSC-derived microglia" was co-authored by Dorit Trudler, Kristopher Nazor, Yvonne Eisele, Titas Grabauskas, Nima Dolatabadi, James Parker, Abdullah Sultan, Zhenyu Zhong, Marshal Goodwin, Yona Levites, Todd Golde, Jeffery Kelly, Michael Sierks, Nicholas Schork, Michael Karin, Rajesh Ambasudhan and Stuart Lipton.

The work was supported by the National Institutes of Health (R01 NS086890, RF1 AG057409, R01 AG056259, R01 DA048882, DP1 DA041722, R01 AI043477).

https://www.eurekalert.org/pub_releases/2021-03/sri-eaf032621.php